The Biochemistry Platform meets the needs of investigators wishing to monitor bone and cartilage biomarkers, including hormones, growth factors and cytokines, in human and rodent body fluids and in cell culture media.

Services

Immunochemical and Radiochemical Assays

(Majority available for human/mouse/rat samples)

• 1,25(OH)2 Vitamin D
• PTH
• PTHrP
• Osteocalcin
• Bone-spec. Alkaline Phosphatase
• C-telopeptides, N-telopeptides
• PINP, PICP
• sRANKL
• TRAP
• Osteoprotegerin
• Matrix Gla Protein
• FGF-23
• IL-6
• IL-1 beta
• TNF alpha
• TGF beta, PDGF, EGF, VEGF, VEGF-D
• IGF-1

In Vitro Bioluminescence (under development)

Protocols are under development to use the Optima microplate reader for kinetic cell-based assays that use Aquorin and Fura-2 to measure intracellular calcium transients.

Technologies

BMG FLUOstar OPTIMA

Combination microplate reader with high-performance luminescence capabilities for measuring fluorescence intensity, time-resolved fluorescence, high-performance luminescence (flash and glow) and absorbance (low UV to visible).

Forms

Download PDF Forms.